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ESET NOD32 Antivirus 3.0.563 FreeThe long-term objectives of this project are to determine how the genome is packaged into chromatin and, in turn, to define the role of chromatin in the regulation of gene expression. The packaging of chromatin into nucleosome arrays requires that DNA-binding proteins, including transcription factors, are spatially organized. Spatial control of gene expression may be mediated by structural changes that alter the accessibility of transcription factors to their target sites on DNA. We will test the hypotheses that: (1) Structural changes in chromatin may affect transcription factor binding by altering accessibility of cis-acting elements to the protein, (2) Spatial organization of regulatory regions may play a role in determining the accessibility of a trans-acting factor to a given cis-acting sequence, and (3) An open chromatin structure may be an essential component of chromatin for function and, in turn, regulate gene expression in developing embryos. Transcription factors are notoriously labile, due to the instability of their associated DNA-binding domains. This instability could result from modification of the protein by covalent modifications (e.g., phosphorylation or acetylation) or altered chromatin structure. The latter could conceivably be in turn modulated by interactions of transcription factors with their cognate regulatory sequences, leading to structural changes that directly affect the protein's stability. To address this question, we will define the relationship between chromatin structure and the transcriptional activity of a transcription factor, GABP, using micrococcal nuclease digestion, chromatin immunoprecipitation, and footprinting techniques in Drosophila embryos. In parallel, we will test the hypothesis that GABP has a specific role in the regulation of a subset of genes, including those required for the development of Drosophila eye, leg, or wing, and that this role may be mediated by the spatial organization of regulatory sequences and GABP binding sites. Such information should provide insight into the role of gene regulation in embryonic development.Q:
How do I change the path of my deployed artifacts on a Jenkins run
I have Jenkins on a server and I want to deploy my artifacts to a local repository on that server.
I have a build step that works fine with SVN, 0b46394aab